活性蛋白 > 其他 > Recombinant Human Programmed cell death 1 ligand 1(CD274),partial (Active)
  • 產品名稱:

    Recombinant Human Programmed cell death 1 ligand 1(CD274),partial (Active) IN STOCK

  • 貨期:
    3-7 工作日
  • uniprot號:
  • 貨號:
    CSB-MP878942HU1
  • 規格:
    ¥1428
  • 圖像:


    (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.



    Activity

    Measured by its binding ability in a functional ELISA. Immobilized PD-L1 at 2 μg/ml can bind Anti- PD-L1 mouse monoclonal antibody(CSB-MA878942A1m,antigen from E.coli), the EC50 of human PD-L1 protein is 1.252-1.653 ng/mL.

  • 測活實驗手冊:
  • 分子結構:
  • 基因名稱:
    CD274
  • 別名:
    PD-L1 (PDCD1 ligand 1) (Programmed death ligand 1) (hPD-L1) (B7 homolog 1) (B7-H1) (CD274)
  • 種屬:
    Homo sapiens (Human)
  • 蛋白描述:
    Partial
  • 分子量:
    52.7 kDa
  • 標簽信息:
    C-terminal FC-tagged
  • 來源:
    Mammalian cell
  • 純度:
    Greater than 95% as determined by SDS-PAGE.
  • 緩沖液:
    Lyophilized from a 0.2 μm filtered PBS, 6% Trehalose, pH 7.4
  • 內毒素:
    Less than 1.0 EU/ug as determined by LAL method.
  • 生物活性:
    Measured by its binding ability in a functional ELISA. Immobilized PD-L1 at 2 μg/ml can bind Anti- PD-L1 mouse monoclonal antibody(CSB-MA878942A1m,antigen from E.coli), the EC50 of human PD-L1 protein is 1.252-1.653 ng/mL.
  • 參考文獻:
    "B7-H1, a third member of the B7 family, co-stimulates T-cell proliferation and interleukin-10 secretion."
    Dong H., Zhu G., Tamada K., Chen L.
    Nat. Med. 5:1365-1369(1999)
  • 氨基酸序列:
    FTVTVPKDLYVVEYGSNMTIECKFPVEKQLDLAALIVYWEMEDKNIIQFVHGEEDLKVQHSSYRQRARLL
    KDQLSLGNAALQITDVKLQDAGVYRCMISYGGADYKRITVKVNAPYNKINQRILVVDPVTSEHELTCQAE
    GYPKAEVIWTSSDHQVLSGKTTTTNSKREEKLFNVTSTLRINTTTNEIFYCTFRRLDPEENHTAELVIPE
    LPLAHPPNER
  • 說明書:

    注:產品說明書可能有改進,請直接與CUSABIO產品專員聯系,索取最新版說明書!

  • 簡介:
    Plays a critical role in induction and maintenance of immune tolerance to self (PubMed:11015443, PubMed:28813417, PubMed:28813410). As a ligand for the inhibitory receptor PDCD1/PD-1, modulates the activation threshold of T-cells and limits T-cell effector response (PubMed:11015443, PubMed:28813417, PubMed:28813410). Through a yet unknown activating receptor, may costimulate T-cell subsets that predominantly produce interleukin-10 (IL10) (PubMed:10581077)
    The PDCD1-mediated inhibitory pathway is exploited by tumors to attenuate anti-tumor immunity and escape destruction by the immune system, thereby facilitating tumor survival (PubMed:28813417, PubMed:28813410). The interaction with PDCD1/PD-1 inhibits cytotoxic T lymphocytes (CTLs) effector function (By similarity). The blockage of the PDCD1-mediated pathway results in the reversal of the exhausted T-cell phenotype and the normalization of the anti-tumor response, providing a rationale for cancer immunotherapy (By similarity).
  • 蛋白儲存:
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
  • 表達區域:
    19-238aa
  • 使用注意:
    Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.
  • 研究領域:
    Cancer